How to extract DNA from different tissue samples with BioMasher series?
What are the filter sizes of BioMasherⅠand III?
1）Product code 893012 (1.5ml tube with O-ring & PP filter): Pore size 80～145μｍ
2）Product code 893041 (1.5ml tube with O-ring & PE filter): Pore size 20～60μm
3）Product code 893131 (PP filter of BioMasher III): Pore size 80～145μｍ
For more information, please see the "User's Manual" P1-5
What are the efficiencies of BioMasher Ⅱ for protein extraction?
Compared with conventional SSI pestle, extraction is more effective with BioMasher Ⅱ.
Please see the "Technical Document"
Can "Biomassher II" be cooled with liquid nitrogen?
The material of the tube is PP, and its freezing resistance is approximately -60℃ ~ -40℃. Cooling of the sample with liquid nitrogen together with the tube may cause damage. If you want to avoid an increase in sample temperature, freeze the material to be homogenized first, then homogenize it on ice.
Are the products RNase free even if they are not sterilized?
We do not perform RNase tests for finished products, so DNase free and RNase free are not guaranteed. Since each factory conducts hygiene management from a number of perspectives, including the flow of goods and human hygiene, DNase or RNase contaminations have not been reported. For customers who require a precondition for DNase free and RNase free, we suggest adding additional clearance processes before using our products.
Are individual parts, such as 1.5ml tubes or pestles for BioMasher II available to purchase?
Individual parts sales are not available.
Can BioMasher II be used for extracting lipid metabolite from an animal organ tissue samples?
After grinding the sample with BioMasher Ⅱ, add a hydrophobic solvent and centrifuge it. This may allow for the recovery of lipid metabolites.
It is stated that homogenizing cultured cells with the "Biomassher II" is not very suitable. What is the reason for this?
Cultured cells are smaller than the grinding surfaces of the BioMasher II, so there is a possibility that they may not be completely homogenized.
For hard tissue such as tumor, are any preparations required before using BioMasher Ⅱ or
BioMasher Ⅲ?
Using samples that have been pre-crush is expected to enable more reliable disruption. Since the Biomassher III is equipped with a filter, fibrous tissues such as tumor tissue may remain on the filter. The use of the Biomassher II is recommended.
What are the filter materials of BioMasher Ⅲ and BioMasher Ⅴ, and what are their hydrophilicity?
The materials of both are polyethylene, and the hydrophilicity is not high.
Can BioMasher series be used for grinding platelets?
The volume of the platelet suspension is approximately 2 ml.
If the filter pore size is larger than the material to be disrupted, efficient disruption is likely to be difficult. Both PP and PE filters have sizes larger than platelets, so it is expected that BioMasher series are not suitable for platelet disruption.
In terms of sample volume, which model of the BioMasher is appropriate?
Our recommendation is as below.
BioMasher II: Sample size 100 mg
BioMasher III: Sample size 50 mg
BioMasher V: Sample size 500mg
BioMasher SP: Sample size 1,000 mg
What are the centrifugal strenghes of the BioMasher series?
The following conditions are standard numbers.
BioMasher I: 10,000-15,000 x g, 10-30 seconds
BioMasher II: 10,000-15,000 x g, 10-30 seconds
BioMasher III: 6,000-10,000 x g, 10-30 seconds
BioMasher V: 3,000-6,000 x g, 5 minutes
BioMasher SP: 3,000-6,000 x g, 5 minutes
Do BioMasher series have resistance to phenol-based reagents?
The following conditions may result from phenol-based reagents of BioMasher SP, for example.
+20℃: No change
+60℃: Possibility of cracks, strength degradations, and discolorations
Do the sterilized items come with a certificate, such as a sterilization certificate?
We have a sterilization certificate, so please feel free to request it.
Can PowerMasher Ⅱ be used for all models of BioMasher series?
PowerMasher II can be used for BioMasher Ⅱ, Ⅲ, Ⅴ, SP（cannot be used for BioMasher I）.
What is an optimal protocol of the PowerMasher II, such as rotation speed, time, and
strength?
The rotation speed of the PowerMasher II is set to 450 rpm, which is relatively low, and as a result, the rotational torque is increased. This allows the rotation to continue even when the material inside the tube is pressed, making it efficient for disrupting hard samples.
The optimal time and strength vary depending on the material being grinded, so we recommend applying for a one-month rental of the PowerMasher II and testing the grinding using BioMasher samples at the same time.