Reagents, Lab Equipment, and Lab Supplies
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Working together with the Nippi Research Institute of Biomatrix, the Biological and Chemical Products Division is responsible for creating innovative basic and applied scientific products such as collagen, laminin, and related products. Our clean room facility also enables us to manufacture reliable, high-quality medical and scientific products.
We distribute various kinds of collagen as reagents, such as a highly purified gelatin MediGelatin for medical use, and "NippiBL BSE Test Kit" for diagnosis of BSE. We have also developed original laboratory devices that are widely accepted domestically and globally, such as the "BioMasher". The Biological and Chemical Products Department continuously aims to contribute globally with our cutting-edge research and innovative, quality development.
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Collagen
Collagen is the most abundant protein in our body, about 30% of total protein. Collagen is rich in bone, skin, tendon, ligament, cartilage, heart, eye, and so on. Synthesis of collagen protein is one of the most complicated processes among all human proteins. Many enzymatically posttranslational modifications involve the synthesis such as 3- and 4-hydroxyproline and 5-hydroxylysine. Some of hydroxylysine residues are additionally modified as galactosyl-hydroxylysine and glucosyl-galactosyl-hydroxylysine. 4-Hydroxylation of proline increases the thermal stability of triple helical structure. These posttranslational modification reactions are essential to the biological function. Defection of these enzymes causes abnormality in bone and other organs. Due to the many posttranslational modifications and the large size of molecule, it is impossible to produce collagen molecules from recombinant expression with reasonable prices. With Nippi’s more than a half century of research expertise, we produce purified collagen from various kinds of animal tissues such as skin, and cornea. Product forms are solution, powder, or sheets of reconstituted fibrils. Types I, III, and V collagens are on our catalog list.
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Low Endotoxin Gelatin
Gelatin is the denatured form of collagen protein. We have medical grade collagen with a very low content of endotoxin (≦10 EU/g). Nippi’s MediGelatin has almost an intact size of the collagen alpha chain, and Nippi’s High Grade Gelatin has a little smaller polypeptide size with MW of 8K on average. Nippi’s technology enables the purification of high-grade gelatin.
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Laminin
Laminin is a major component of basement membrane. Our series of iMatrix products are the recombinant E8 fragment of human laminin protein with chemically defined animal component-free medium. iMatrix-511, recombinant human laminin-511 E8 fragment, is an excellent substrate for maintaining ES/iPS cells. One of the major benefits of our iMatrix-511 is the ability for the single cell passaging of iPS/ES cells. iMatrix-411 and -221 are good substrates for endothelial cells and cardiomyocytes. We also have silkworm filament-derived inexpensive edition of iMatrix-511.
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MatriMix 3D cell culture substrate
MatriMix is a substrate for 3D cell culture consisting of collagen, hyaluronic acid, and laminin. MatriMix is in a solution state at low temperature but becomes a gel when heated for culture. By combining collagen types (I, II, III, IV, V, etc.) and laminin isoforms, you can construct a 3D cell culture environment suitable for a variety of cells. For more information, please refer to the MatriMix product website below.
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Lab Equipment and Supplies
BioMasher is a single-use homogenizer for nucleic acid extraction, protein extraction, and cell disruption. We have a battery powered motor, PowerMasher, designed for BioMasher, long tips, and racks, etc.
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Collagen-related reagent
As a collagen-related reagent, we have developed a new high-purity collagen-degrading enzyme and aim to apply it to medical applications as well as to use it as reagent. Realized low endotoxin and free of animal-derived ingredients.
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BSE（Bovine Spongiform Encephalopathy）test kit
From 1996, we have collaborated with National Institute of Animal Health and the National Agriculture and Food Research Organization to study prion protein. We developed a highly sensitive BSE test kit. Our kit has been widely used all over Japan.
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